Purpose: To ensure consistent and effective cleaning and disinfection of cleanroom floors using sterile mopping methods.
Materials and Equipment
- Sterile mop head (pre-saturated single-use mop wipes for a bucketless system).
- Mop handle compatible with sterile heads.
- Product:
- Detergent solution for residue removal
- DE 70% v/v for disinfection
- Personal protective equipment (PPE) according to cleanroom requirements.
Procedure
1. Preparation
- Don appropriate PPE.
- Prepare sterile mop head according to manufacturer instructions.
2. Mopping Technique
- Begin in the cleanest area of the room, working toward the dirtiest areas or designated exit point.
- Use parallel strokes that overlap slightly; avoid circular motions to prevent re-deposition of contaminants.
- Pull the mop toward you, back to front for horizontal surfaces (floor), lifting the mop head at the end of each stroke.
- Maintain consistent direction (left-to-right or right-to-left) throughout the cleaning session.
Solution and Mop Head Management
- Use appropriate solution based on task:
- Detergent for general cleaning
- DE 70% v/v for disinfection
- Sporicidal agent as scheduled
- Replace mop head when visibly soiled or after reaching pre-defined usage limit (e.g., every 10-15 sq. ft. or at a set interval) to avoid dragging contamination.
Safety and Compliance
- Avoid over-saturating surfaces.
- Follow all cleanroom handling protocols.
- Ensure proper labelling and storage of cleaning agents.
Critical Rules
- Always use distilled, deionised, or sterile water to prevent mineral buildup.
- Unidirectional Movement: Use overlapping, unidirectional strokes. This ensures contaminants are picked up and not just spread around.
- Slow Movements: Keep movements slow and controlled to avoid making particles airborne.
- Ensure the disinfectant remains wet on the surface for the time recommended by the manufacturer.
Download PDF
Download PDF with mopping illustration:
AGMA sterile mop wipe technique (bucketless system)

